Journal
BIOPHYSICAL JOURNAL
Volume 96, Issue 10, Pages 4268-4275Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2009.02.045
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Funding
- STREP BIOMACH [NMP4-CT-2003-505-487, ANR-05-NANO-045]
- Fondation pour la Recherche Medicale
- National Institutes of Health [GM33289]
- American Heart Association
- Burroughs Wellcome Fund
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We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an intracellular measurement of key parameters of this molecular transporter: velocity, processivity, step size, and dwell time. Our experiments bridge the gap between in vitro single molecule assays and the indirect measurements of the motor features deduced from the tracking of organelles in live cells.
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