Journal
BIOPHYSICAL JOURNAL
Volume 96, Issue 9, Pages 3629-3637Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2009.01.045
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Funding
- Ministerio de Ciencia e Innovacion [FIS2006-01305]
- Consolider Ingenio en Nanociencia Molecular [CSD2007-0010]
- Comunidad de Madrid [S-050/MAT/0283]
- Deutsche Forschungsgemeinschaft [Sfb 448, TP A12]
- European Union
- NANOBIO-M [CAM S-0505/MAT/0283]
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In this study, the center-of-mass diffusion and shape fluctuations of large unilamellar 1-palmitoyl-2-oleyl-sn-glycero-phosphatidylcholine vesicles prepared by extrusion are studied by means of neutron spin echo in combination with dynamic light scattering. The intermediate scattering functions were measured for several different values of the momentum transfer, q, and for different cholesterol contents in the membrane. The combined analysis of neutron spin echo and dynamic light scattering data allows calculation of the bending elastic constant, kappa, of the vesicle bilayer. A stiffening effect monitored as an increase of kappa with increasing cholesterol molar ratio is demonstrated by these measurements.
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