Journal
BIOPHYSICAL JOURNAL
Volume 96, Issue 7, Pages 2912-2917Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2008.12.3945
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Funding
- BBSRC [BB/E019668/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E019668/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/D001846/1] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/E019668/1] Funding Source: Medline
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We apply the astronomical data-analysis technique, Lucky imaging, to improve resolution in single molecule fluorescence microscopy. We show that by selectively discarding data points from individual single-molecule trajectories, imaging resolution can be improved by a factor of 1.6 for individual fluorophores and up to 5.6 for more complex images. The method is illustrated using images of fluorescent dye molecules and quantum dots, and the in vivo imaging of fluorescently labeled linker for activation of T cells.
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