Journal
BIOPHYSICAL JOURNAL
Volume 96, Issue 1, Pages 86-100Publisher
CELL PRESS
DOI: 10.1529/biophysj.108.136242
Keywords
-
Categories
Funding
- The Netherlands Organization of Scientific Research [NWO 047.017.034]
- Agence Nationale de Recherche
- National Sciences and Engineering Research Council of Canada
Ask authors/readers for more resources
Ampullosporin A and alamethicin are two members of the peptaibol family of antimicrobial peptides. These compounds are produced by fungi and are characterized by a high content of hydrophobic amino acids, and in particular the alpha-tetrasubstituted amino acid residue a-aminoisobutyric acid. Here ampullosporin A and alamethicin were uniformly labeled with N-15, purified and reconstituted into oriented phophatidylcholine lipid bilayers and investigated by proton-decoupled N-15 and (31)p solid-state NMR spectroscopy. Whereas alamethicin (20 amino acid residues) adopts transmembrane alignments in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes the much shorter ampullosporin A (115 residues) exhibits comparable configurations only in thin membranes. In contrast the latter compound is oriented parallel to the membrane surface in 1,2-dimyristoleoyl-sn-glycero-3-phosphocholine and POPC bilayers indicating that hydrophobic mismatch has a decisive effect on the membrane topology of these peptides. Two-dimensional N-15 chemical shift - H-1-N-15 dipolar coupling solid-state NMR correlation spectroscopy suggests that in their transmembrane configuration both peptides adopt mixed alpha-/3(10)-helical structures which can be explained by the restraints imposed by the membranes and the bulky alpha-aminoisobutyric acid residues. The N-15 solid-state NMR spectra also provide detailed information on the helical tilt angles. The results are discussed with regard to the antimicrobial activities of the peptides.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available