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Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

Journal

EMERGING MICROBES & INFECTIONS
Volume 9, Issue 1, Pages 1393-1396

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/22221751.2020.1775500

Keywords

SARS-CoV-2; COVID 19; rt-Real time PCR; molecular methods; heat treatment; nasopharyngeal samples

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The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

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