Journal
BIOPHYSICAL JOURNAL
Volume 96, Issue 1, Pages L1-L3Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2008.09.032
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We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.
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