Journal
BIOPHYSICAL CHEMISTRY
Volume 182, Issue -, Pages 64-70Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bpc.2013.06.015
Keywords
Cholesterol-dependent cytolysin; Listeriolysin O; Perfringolysin O; Planar lipid membranes; Pore-forming toxin; Pore
Funding
- Slovenian Research Agency
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Pore-forming toxins are an important group of natural molecules that damage cellular membranes by forming transmembrane pores. They are used by many organisms for attack or defense and similar proteins are employed in the immune system of vertebrates. Various biophysical approaches have been used to understand how these proteins act at the molecular level. One of the most useful, in terms of monitoring pore formation in real time, is a method that employs planar lipid membranes and involves ionic current measurements. Here we highlight the advantages and possibilities that this approach offers and show how it can advance understanding of the pore-forming mechanism and pore properties for one of the most important families of natural toxins, the cholesterol-dependent cytolysins. (c) 2013 Elsevier B.V. All rights reserved.
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