Journal
BIOORGANIC CHEMISTRY
Volume 44, Issue -, Pages 1-7Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bioorg.2012.06.002
Keywords
Fluorometabolites; Fluoroacetate; 4-Fluorothreonine; Streptomyces cattleya; Biosynthesis; Genome sequence
Funding
- BBSRC [BB/F007426/1]
- 973 Program, Ministry of Science and Technology, China [2009CB118901, 2012CB721002]
- National Natural Science Foundation of China
- Program for New Century Excellent Talents in University, Ministry of Education, China [NCET-10-0572]
- Chen Xing Young Scholars Program
- BBSRC [BB/F007426/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F007426/1] Funding Source: researchfish
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Streptomyces cattleya DSM 46488 is unusual in its ability to biosynthesise fluorine containing natural products, where it can produce fluoroacetate and 4-fluorothreonine. The individual enzymes involved in fluorometabolite biosynthesis have already been demonstrated in in vitro investigations. Candidate genes for the individual biosynthetic steps were located from recent genome sequences. In vivo inactivation of individual genes including those encoding the S-adenosyl-L-methionine: fluoride adenosyltransferase (fluorinase, SCATT_41540), 5'-fluoro-5'-deoxyadenosine phosphorylase (SCATT_41550), fluoroacetyl-CoA thioesterase (SCATT_41470), 5-fluoro-5-deoxyribose-1-phosphate isomerase (SCATT_20080) and a 4-fluorothreonine acetaldehyde transaldolase (SCATT_p11780) confirm that they are essential for fluorometabolite production. Notably gene disruption of the transaldolase (SCATT_p11780) resulted in a mutant which could produce fluoroacetate but was blocked in its ability to biosynthesise 4-fluorothreonine, revealing a branchpoint role for the PLP-transaldolase. (C) 2012 Elsevier Inc. All rights reserved.
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