Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 24, Issue 18, Pages 4533-4537Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2014.07.074
Keywords
Bisecting GlcNAc; N-Glycan; Synthesis; Glycosylation; N-Acetylglucosaminyltransferase III
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Funding
- Japan Society of the Promotion of Science (JSPS) [24710257, 25460054]
- Tokyo Biochemical Research Foundation
- RIKEN Fund
- Grants-in-Aid for Scientific Research [25460054, 24710257, 26440051] Funding Source: KAKEN
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N-Acetylglucosaminyltransferase (GnT) III is a glycosyltransferase which produces bisected N-glycans by transferring GlcNAc to the 4-position of core mannose. Bisected N-glycans are involved in physiological and pathological processes through the functional regulation of their carrier proteins. An understanding of the biological functions of bisected glycans will be greatly accelerated by use of specific inhibitors of GnT-III. Thus far, however, such inhibitors have not been developed and even the substrate-binding mode of GnT-III is not fully understood. To gain insight into structural features required of the substrate, we systematically synthesized four N-glycan units, the branching parts of the bisected and non-bisected N-glycans. The series of syntheses were achieved from a common core trimannose, giving bisected tetra-and hexasaccharides as well as non-bisected tri- and pentasaccharides. A competitive GnT-III inhibition assay using the synthetic substrates revealed a vital role for the Man beta(1-4) GlcNAc moiety. In keeping with previous reports, GlcNAc at the alpha 1,3-branch is also involved in the interaction. The structural requirements of GnT-III elucidated in this study will provide a basis for rational inhibitor design. (C) 2014 Elsevier Ltd. All rights reserved.
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