Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS
Volume 35, Issue 3, Pages 83-89Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/1724600820951572
Keywords
miR-183-5p; IRS1; hepatocellular carcinoma; cell proliferation; prognosis
Categories
Funding
- National Natural Science Foundation of China [81370069/H1617]
- Natural Science Foundation of First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Shaanxi, China [2017QN-04]
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Background: MiR-183-5p plays an important role in the pathophysiology of many tumors, while the role of MiR-183-5p in liver cancer is unclear. Methods: In this study, quantitative reverse transcription-polymerase chain reaction and Western blotting were used to detect the expression of miR-183-5p in liver cancer cell lines, liver cancer tissues, and normal tissues adjacent to the cancer, and to explore the mechanism of miR-183-5p regulating liver cancer progression. The in vitro effects of miR-183-5p were evaluated by CCK-8, colony formation test, and wound healing test. Various databases were used to predict the target mRNA of miR-183-5p and verified by luciferase report analysis. In addition, the effects of miR-183-5p and its target gene on the survival of patients with liver cancer were also analyzed. Results: miR-183-5p was highly expressed in hepatocellular carcinoma cells and tissues, and was related to some clinicopathological features. MiR-183-5p can promote the proliferation and migration of liver cancer cells. Using the bioinformatics database, we proved that miR-183-5p is related to the survival of liver cancer patients. Insulin receptor substrate 1 (IRS1) is a target of miR-183-5p, and luciferase analysis confirmed that miR-183-5p combines with the 3 '-untranslated region (3 '-UTR) of IRS1. Conclusion: The miR-183-5p/IRS1 axis may be a new target for liver cancer research.
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