Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 21, Issue 23, Pages 7175-7179Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2011.09.070
Keywords
Dinucleoside polyphosphate; Ap(4)A; Bio-panning; Magnetic bead; Proteomics
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Funding
- Natural Science Foundation of China [30825045]
- Chinese Scholarship Council
- British Council
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We report the development of a synthetic, biotin-conjugated diadenosine tetraphosphate (Ap(4)A)-'molecular hook' attached to magnetic beads enabling the isolation of Ap(4)A-binding proteins from bacterial cells or mammalian tissue lysates. Characterisation and identification of isolated binding proteins is performed sequentially by mass spectrometry. The observation of positive controls suggests that these newly observed proteins are putative Ap(4)A-binding partners, and we have expectations that others can be found with further technical improvements in our methods. (C) 2011 Elsevier Ltd. All rights reserved.
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