Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 21, Issue 15, Pages 4503-4506Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2011.05.120
Keywords
Aptamer; Ribonucleopeptide; Biosensor; Modular design; Fluorescence detection
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Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan [20241051, 22121510]
- Grants-in-Aid for Scientific Research [20241051, 22121510, 21710232] Funding Source: KAKEN
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A GTP aptamer was converted to a modular fluorescent GTP sensor by conjugation of RRE (Rev responsive element) RNA and successive complex formation with a fluorophore-modified Rev peptide. Structural changes associated with substrate binding in the RNA aptamer were successfully transduced into changes in fluorescence intensity because of the modular structure of ribonucleopeptides. A simple modular strategy involving conjugation of a fluorophore-modified ribonucleopeptide to the stem region of an RNA aptamer deduced from secondary structural information helps produce fluorescent sensors, which allow tuning of excitation and detection wavelengths through the replacement of the fluorophore at the N-terminal of the Rev peptide. (C) 2011 Elsevier Ltd. All rights reserved.
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