Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 21, Issue 13, Pages 3931-3934Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2011.05.023
Keywords
Luciferin; Carboxypeptidase A; Carboxypeptidase G; Luciferase; Glutamic acid; Tyrosine
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Funding
- NIH [CA135312]
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Highly selective luminescent probes, QLUC-TYR and LUC-GLU, for detection of carboxypeptidase activity were synthesized. Caged substrates were first cleaved by corresponding carboxypeptidases, and then they were activated by luciferase to emit light. Enzymatic activities of biologically important carboxypeptidases can be determined using this technology. (C) 2011 Elsevier Ltd. All rights reserved.
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