Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 21, Issue 17, Pages 4945-4950Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2011.05.045
Keywords
Metabolic labeling; Glycoproteomics; Prostate cancer; Azidosugar; Tissue slice cultures
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Funding
- National Institutes of Health [GM66047, U01CA128416]
- Department of Defense [PC080659]
- National Science Foundation
- Howard Hughes Medical Institute
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Most clinically approved biomarkers of cancer are glycoproteins, and those residing on the cell surface are of particular interest in biotherapeutics. We report a method for selective labeling, affinity enrichment, and identification of cell-surface glycoproteins. PC-3 cells and primary human prostate cancer tissue were treated with peracetylated N-azidoacetylgalactosamine, resulting in metabolic labeling of cell surface glycans with the azidosugar. We used mass spectrometry to identify over 70 cell surface glycoproteins and biochemically validated CD146 and integrin beta-4, both of which are known to promote metastatic behavior. These results establish cell-surface glycoproteomics as an effective technique for discovery of cancer biomarkers. (C) 2011 Published by Elsevier Ltd.
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