Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 20, Issue 22, Pages 6565-6568Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2010.09.044
Keywords
Locked nucleic acid; Modified nucleotides; Functional nucleic acids; Enzymatic incorporation
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Funding
- Danish National Research Foundation
- Danish National Advance Technology Foundation
- Idaho NSF EPSCoR
- BANTech Center at the University of Idaho
- University of Idaho Research Office
- Research Council
- University of Queensland
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Modified nucleic acids have considerable potential in nanobiotechnology for the development of nanomedicines and new materials. Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far and we herein for the first time report the enzymatic incorporation of LNA-U and C5-ethynyl LNA-U nucleotides into oligonucleotides. Phusion High Fidelity and KOD DNA polymerases efficiently incorporated LNA-U and C5-ethynyl LNA-U nucleotides into a DNA strand and T7 RNA polymerase successfully accepted the LNA-U nucleoside 50-triphosphate as substrate for RNA transcripts. (C) 2010 Elsevier Ltd. All rights reserved.
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