Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 36, Issue 8, Pages 1464-1476Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0271678X15611137
Keywords
Ischemic stroke; lymphocytes; CD4(+) T cells; USPIO; In vivo imaging
Categories
Funding
- National Basic Research Program of China [2013CB966900]
- National Key-Project of Clinical Neurology
- National Science Foundation of China [81230028, 81301044, 81471535]
- American Heart Association [GRNT18970031]
- National Institutes of Health [R01AI083294]
- PhD Programs Foundation of Ministry of Education of China [20121202120007]
- Tianjin Education Commission Foundation [20120116, 14JCYBJC42000]
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Recent studies have demonstrated that lymphocytes play a key role in ischemic brain injury. However, there is still a lack of viable approaches to non-invasively track infiltrating lymphocytes and reveal their key spatiotemporal events in the inflamed central nervous system (CNS). Here we describe an in vivo imaging approach for sequential monitoring of brain-infiltrating CD4(+) T cells in experimental ischemic stroke. We show that magnetic resonance imaging (MRI) or Xenogen imaging combined with labeling of SPIO-Molday ION Rhodamine-B (MIRB) can be used to monitor the dynamics of CD4(+) T cells in a passive transfer model. MIRB-labeled CD4(+) T cells can be longitudinally visualized in the mouse brain and peripheral organs such as the spleen and liver after cerebral ischemia. Immunostaining of tissue sections showed similar kinetics of MIRB-labeled CD4(+) T cells when compared with in vivo observations. Our results demonstrated the use of MIRB coupled with in vivo imaging as a valid method to track CD4(+) T cells in ischemic brain injury. This approach will facilitate future investigations to identify the dynamics and key spatiotemporal events for brain-infiltrating lymphocytes in CNS inflammatory diseases.
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