Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 20, Issue 16, Pages 4906-4910Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2010.06.066
Keywords
N-Acetylglucosamine; N-Acetyllactosamine; Sialic acid; Sialyllactosamine; Glycosides; Oligosaccharides; Clusters; Chemo-enzymatic syntheses; Glycosyltransferases
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Funding
- Ministry of Health, Labor, and Welfare of Japan [14-N-O15]
- Grants-in-Aid for Scientific Research [21550152] Funding Source: KAKEN
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An efficient synthesis of sialyllactosamine (SiaLacNAc) clusters using carbosilanes as core scaffolds has been accomplished by means of chemical and enzymatic approaches. N-Acetyl-D-glucosamine (GlcNAc) clusters having O-glycosidic linkage or S-glycosidic linkage were chemically synthesized from known intermediates in high yields. The GlcNAc clusters were first used as substrates for beta 1,4 galactosyl transferase using UDP-galactose (UDP-Gal) as a sugar source to provide corresponding N-acetyllactosamine clusters. Further sugar elongation of the LacNAc clusters was demonstrated using alpha 2,3 sialyl transferase and CMP-neuraminic acid (CMP-NANA) to yield the corresponding SiaLacNAc clusters. (c) 2010 Elsevier Ltd. All rights reserved.
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