Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 19, Issue 23, Pages 6585-6587Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2009.10.039
Keywords
LNA; 2 '-Amino-LNA; Aptamer; Surface plasmon resonance; Affinity
Categories
Funding
- Danish National Research Foundation
- Danish Agency for Science Technology and Innovation
- Nucleic Acid Based Drug Design (NAC-DRUG)
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The affinity change upon incorporation of LNA and 2'-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2'-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities. (C) 2009 Elsevier Ltd. All rights reserved.
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