Journal
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
Volume 13, Issue 3, Pages 952-966Publisher
E-CENTURY PUBLISHING CORP
Keywords
Calcium phosphate cement; lithium; aspirin; inflammation; osteoporotic bone defect
Categories
Funding
- University Natural Science Research Project of Anhui Province [KJ2017A266, KJ2017A117]
- Panfeng Innovation Team Project for Scientific Research of Yijishan Hospital, Wannan Medical College [GF2019G04, PF2019005, GF2019T02, PF2019007]
- National Natural Science Foundation of China [82002322]
- Wannan Medical College [YR201917]
- Young and Middle-aged Key Project of Wannan Medical College [WK2020ZF16]
- Additive Manufacturing Institute of Anhui Polytechnic University Open Project [2020ybxm06]
- Wuhu Science and Technology Plan Project [2020ms3-1]
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Local application of lithium or aspirin with biological scaffold has been shown to improve bone formation. In this study, Asp-Li/CPC was prepared and confirmed to have better ability in promoting osteoblast differentiation and mineralization. In vivo and in vitro experiments demonstrated that Asp-Li/CPC can rapidly repair bone defects by inhibiting local inflammation and activating specific signaling pathways.
Local application of lithium or aspirin with biological scaffold has been identified as a potent means to improve bone formation. In this study, lithium and aspirin modified calcium phosphate cement (Asp-Li/CPC) was prepared, and the feasibility of this biological scaffold in the treatment of osteoporotic bone defect was observed in vivo and in vitro. In vitro experiments confirmed that Asp-Li/CPC had better ability to promote MC3T3-E1 cells differentiation into osteoblasts, osteoblast mineralization and viability, and promote cell expression of ALP, OP, RUNX-2, OC and COL-1 protein than simple CPC or lithium modified CPC by MTT, Alizarin red staining and Western blot evaluation. In vivo experiments confirmed that Asp-Li/CPC presented the strongest effect on bone regeneration and bone mineralization through the comparison with CPC group and Li/CPC group with X-ray images, Micro-CT and Histological evaluation. RT-qPCR analysis showed that Asp-Li/CPC, Li/CPC group and CPC group demonstrated increased BMP2, Smad1, OPG than the OVX group (P<0.05), while Asp-Li/CPC exhibited decreased TNF-alpha, IFN-gamma and RANKL than the OVX group (P<0.05). Experiments in vivo and in vitro show that Asp-Li/CPC is a scheme for rapid repair of femoral condylar defects, and these effects may be achieved by inhibiting local inflammation and through BMP-2/Smad1 and OPG/RANKL signaling pathway.
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