Hyperosmolarity-Induced Down-Regulation of Claudin-2 Mediated by Decrease in PKC-Dependent GATA-2 in MDCK Cells

Hyperosmolarity decreases claudin-2 expression in renal tubular epithelial cells, but the molecular mechanism remains undefined. Here, we found that the hyperosmolarity-induced decrease in claudin-2 expression is inhibited by Go6983, a non-selective protein kinase C (PKC) inhibitor, and PKC specific inhibitor in Madin-Darby canine kidney II cells. Hyperosmolarity increased intracellular free Ca2+ concentration and phosphorylated PKC level, which were inhibited by RN-1734, an antagonist of transient receptor potential vanilloid 4 channel. Phorbol 12-myristate 13-acetate, a PKC activator, decreased claudin-2 expression. These results indicate hyperosmolarity decreases claudin-2 expression mediated by the activation of RN-1734-sensitive channel and PKC. Hyperosmolarity decreased promoter activity of claudin-2, which was inhibited by Go6983 and PKC inhibitor similar to those in real-time PCR and Western blotting. The effect of hyperosmolarity on promoter activity was not observed in the construct of -469/-6, a deletion mutant. Claudin-2 has hyperosmolarity-sensitive region in its promoter, which includes GATA binding site. Hyperosmolarity decreased the nuclear level of GATA-2, which was inhibited by Go6983 and PKC inhibitor. Mutation of GATA binding site decreased the basal promoter activity and inhibited the effect of hyperosmolarity. In contrast, the hyperosmolarity-induced decrease in reporter activity and claudin-2 expression were rescued by over-expression of wild type GATA-2. Chromatin immunoprecipitation assay showed that GATA-2 bound to promoter region of claudin-2. These results suggest that hyperosmolarity decreases the expression level of claudin-2 via a decrease in PKC-dependent GATA-2 transcriptional activity in renal tubular epithelial cells. J. Cell. Physiol. 9999: 2776-2787, 2015. (c) 2015 Wiley Periodicals, Inc.