Sex chromosomes in the Vizcacheras' White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)

Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA 3/DAPI) and fluorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA 3 positive (DAPI negative) at fluorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fifth within the speciose family Leptodactylidae.

Automated summary

Cytogenetic analyses on Leptodactylus bufonius in Argentina revealed a karyotype of 2n = 2x = 22 and FN = 44, with secondary constrictions in chromosome pair 8 and a sex-linked pericentromeric band in chromosome pair 4. The presence of heteromorphic XY sex chromosomes in L. bufonius is the second case within the genus Leptodactylus and the fifth within the family Leptodactylidae.