Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 22, Issue 3, Pages 1003-1007Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2013.12.044
Keywords
Antisense oligonucleotide; microRNA; RISC; Psoralen; Photoreactive
Funding
- Ministry of Education, Science, Sports and Culture of Japan [2455018600, 2562013200]
- Kyoto-Funding for Innovation in Health-related RD Fields [2013-83]
- [2011B05]
- [2013B08]
- Grants-in-Aid for Scientific Research [25620132, 26410179] Funding Source: KAKEN
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We developed a novel method for regulation of RISC function by photoreactive oligonucleotides (Ps-Oligo) containing 2'-O-psoralenylmethoxyethyl adenosine (Aps). We observed that inhibitory effects of Ps-Oligos on RISC function were enhanced by UV-irradiation compared with 2'-O-methyl-oligonucleotide without Aps. These results suggest Ps-Oligo inhibited RISC function by cross-linking effect, and we propose that the concept described in this report may be promising and applicable one to regulate the small RNA-mediated post-transcriptional regulation. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
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