Journal
ENDOCRINOLOGY
Volume 162, Issue 11, Pages -Publisher
ENDOCRINE SOC
DOI: 10.1210/endocr/bqab186
Keywords
leiomyoma; MIAT; miR-29; TGF-beta 3; MED12 mutation
Categories
Funding
- National Institutes of Health [HD088868, HD100529, HD101852]
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The lncRNA MIAT plays a crucial role in leiomyoma pathogenesis by regulating the expression of the miR-29 family, leading to downstream effects on collagen and TGF-β3. These findings highlight the physiological significance of MIAT in extracellular matrix accumulation in leiomyoma.
The objective of this study was to determine the expression and functional role of a long noncoding RNA (lncRNA) MIAT (myocardial infarction-associated transcript) in leiomyoma pathogenesis. Leiomyoma compared with myometrium (n = 66) expressed significantly more MIAT that was independent of race/ethnicity and menstrual cycle phase but dependent on MED12 (mediator complex subunit 12) mutation status. Leiomyomas bearing the MED12 mutation expressed higher levels of MIAT and lower levels of microRNA 29 family (miR-29a, -b, and -c) compared with MED12 wild-type leiomyomas. Using luciferase reporter activity and RNA immunoprecipitation analysis, MIAT was shown to sponge the miR-29 family. In a 3-dimensional spheroid culture system, transient transfection of MIAT siRNA in leiomyoma smooth muscle cell (LSMC) spheroids resulted in upregulation of miR-29 family and downregulation of miR-29 targets, collagen type I (COL1A1), collagen type III (COL3A1), andTGF-beta 3 (transforming growth factor beta-3). Treatment of LSMC spheroids with TGF-beta 3 induced COL1A1, COL3A1, and MIAT levels, but repressed miR-29 family expression. Knockdown of MIAT in LSMC spheroids blocked the effects of TGF-beta 3 on the induction of COL1A1 and COL3A1 expression. Collectively, these results underscore the physiological significance of MIAT in extracellular matrix accumulation in leiomyoma.
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