Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 21, Issue 11, Pages 2832-2842Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2013.04.008
Keywords
High-throughput lectin aggregation assay; Lanthanides; Luminescence; Nanoparticles
Funding
- Japan Science and Technology Agency (JST)
- Japan Society for the Promotion of Science (JSPS) [24710248]
- SBI Pharmaceuticals Co., Ltd
- Grants-in-Aid for Scientific Research [24710248, 24550148] Funding Source: KAKEN
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To create a novel high-throughput lectin assay (HTPLA) method based on the emission of a luminophore by highly penetrable near-infrared excitation, sugar-attached upconversion lanthanide nanoparticles (LNPs) were synthesized as a tool to highlight the aggregates caused by the sugar-mediated specific bridging between LNP and lectin. The emissions from a mannose-coated LNP in the aggregates with a mannose-binding lectin were much stronger than those from the non-aggregated samples, being sensitive enough for HTPLA. A galactose-coated LNP was also applicable to a macrophage aggregation assay for the sugar specificity of its surface lectin. (C) 2013 Elsevier Ltd. All rights reserved.
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