4.5 Article

Systematic evaluation of SARS-CoV-2 antigens enables a highly specific and sensitive multiplex serological COVID-19 assay

Related references

Note: Only part of the references are listed.
Article Microbiology

COVID-19 Serology at Population Scale: SARS-CoV-2-Specific Antibody Responses in Saliva

Pranay R. Randad et al.

Summary: This study found that salivary antibodies could be a noninvasive alternative for monitoring SARS-CoV-2 infection and seropositivity at a population scale. The results showed a high correlation between salivary and serum antibodies, and high sensitivity and specificity of salivary antibodies for detecting COVID-19 infection.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

Article Cell Biology

IgA dominates the early neutralizing antibody response to SARS-CoV-2

Delphine Sterlin et al.

Summary: The study found that humoral immune responses to SARS-CoV-2 infection are dominated by IgA antibodies, with specific IgA plasmablasts rapidly expanding shortly after symptom onset and peaking in the third week of the disease. Virus-specific antibody responses include IgG, IgM, and IgA, with IgA playing a greater role in virus neutralization compared to IgG.

SCIENCE TRANSLATIONAL MEDICINE (2021)

Article Immunology

Development and Validation of a Multiplex, Bead-based Assay to Detect Antibodies Directed Against SARS-CoV-2 Proteins

Robert A. Bray et al.

Summary: This study introduces a high-throughput multiplex antibody detection assay for identifying patient responses to 5 SARS-CoV-2 proteins. The assay is specific to SARS-CoV-2 antibodies and can be used effectively in transplant settings. It provides a novel tool for understanding immune responses to SAR-CoV-2.

TRANSPLANTATION (2021)

Article Microbiology

Assessment of S1-, S2-, and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays

Georg Semmler et al.

Summary: This study found that differences in sensitivity among commercial immunoassays during acute SARS-CoV-2 infection are only partly related to the target antigen. NCP-specific IgA and IgG antibodies are detected earlier, while higher levels of S1-specific IgA antibodies occur in severely ill patients.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

Article Virology

Longitudinal follow-up of IgG anti-nucleocapsid antibodies in SARS-CoV-2 infected patients up to eight months after infection

Jan Van Elslande et al.

Summary: The study found that antibody levels against SARS-CoV-2 decreased gradually after 2 months, with significantly higher levels in severe patients compared to mild cases. 70.6% of severe patients remained antibody-positive up to eight months after infection, while 61.1% of mild patients became seronegative within six months.

JOURNAL OF CLINICAL VIROLOGY (2021)

Article Multidisciplinary Sciences

A multiplex chemiluminescent immunoassay for serological profiling of COVID-19-positive symptomatic and asymptomatic patients

Allison N. Grossberg et al.

Summary: This study presents results from a multiplex serology test for assessing COVID-19 antibody responses, showing distinct dynamics of IgG, IgM, and IgA responses in patients with different disease severity. It suggests that antibody responses to SARS-CoV-2 may serve as important biomarkers for assessing the risk of viral transmission.

NATURE COMMUNICATIONS (2021)

Article Multidisciplinary Sciences

Evolution of immune responses to SARS-CoV-2 in mild-moderate COVID-19

Adam K. Wheatley et al.

Summary: The durability of infection-induced SARS-CoV-2 immunity has major implications for reinfection and vaccine development. Antibody, B cell, and T cell responses decline over the first 4 months post-infection, while S-specific IgG(+) memory B cells consistently accumulate. The study suggests that natural infection may only provide transient protection at a population level, highlighting the need for more immunogenic and durable vaccines.

NATURE COMMUNICATIONS (2021)

Article Cell Biology

Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients

Yang Li et al.

Summary: By analyzing the serum IgG response of 1,051 COVID-19 patients, researchers identified linear epitopes of the SARS-CoV-2 Spike protein, revealing two regions rich in linear epitopes and identifying variable peptide responses associated with disease severity. Antibodies obtained from immunizing mice with linear epitopes did not show significant neutralizing activity against the virus, suggesting the importance of understanding humoral responses for vaccine refinement.

CELL REPORTS (2021)

Review Chemistry, Multidisciplinary

COVID-19 Antibody Tests and Their Limitations

Guoqiang Liu et al.

Summary: COVID-19 pandemic is a global health crisis with over 2 million deaths, where the main diagnostic methods are nucleic acid PCR tests, viral antigen tests, and human antibody tests. The limitations of antibody tests include inaccuracies due to various factors such as testing windows, individual variances, and fluctuation in antibody levels.

ACS SENSORS (2021)

Article Infectious Diseases

Multiplex assays for the identification of serological signatures of SARS-CoV-2 infection: an antibody-based diagnostic and machine learning study

Jason Rosado et al.

Summary: This study developed a multiplex serological assay to accurately classify individuals with previous SARS-CoV-2 infection based on antibody responses to multiple antigens. The findings provide a potential solution to measuring seroprevalence levels in low-transmission settings and for classifying individuals infected over 6 months ago.

LANCET MICROBE (2021)

Letter Infectious Diseases

An interactive web-based dashboard to track COVID-19 in real time

Ensheng Dong et al.

LANCET INFECTIOUS DISEASES (2020)

Article Multidisciplinary Sciences

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Peng Zhou et al.

NATURE (2020)

Article Multidisciplinary Sciences

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

Daniel Wrapp et al.

SCIENCE (2020)

Review Medicine, General & Internal

COVID-19 diagnosis and management: a comprehensive review

Giuseppe Pascarella et al.

JOURNAL OF INTERNAL MEDICINE (2020)

Review Pathology

Review of Current Advances in Serologic Testing for COVID-19

Andrea P. Espejo et al.

AMERICAN JOURNAL OF CLINICAL PATHOLOGY (2020)

Review Immunology

Overview of Immune Response During SARS-CoV-2 Infection: Lessons From the Past

Vibhuti Kumar Shah et al.

FRONTIERS IN IMMUNOLOGY (2020)

Article Medicine, General & Internal

False Positive COVID-19 Antibody Test in a Case of Granulomatosis With Polyangiitis

Argyrios Tzouvelekis et al.

FRONTIERS IN MEDICINE (2020)

Letter Biochemical Research Methods

COVIDep: a web-based platform for real-time reporting of vaccine target recommendations for SARS-CoV-2

Syed Faraz Ahmed et al.

NATURE PROTOCOLS (2020)

Review Immunology

Vaccines for COVID-19

J. S. Tregoning et al.

CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2020)

Review Medicine, General & Internal

Diagnostic accuracy of serological tests for covid-19: systematic review and meta-analysis

Mayara Lisboa Bastos et al.

BMJ-BRITISH MEDICAL JOURNAL (2020)

Article Immunology

Are Seroprevalence Estimates for Severe Acute Respiratory Syndrome Coronavirus 2 Biased?

Saki Takahashi et al.

JOURNAL OF INFECTIOUS DISEASES (2020)

Article Multidisciplinary Sciences

Neuropilin-1 is a host factor for SARS-CoV-2 infection

James L. Daly et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden

Ann-Sofie Rudberg et al.

NATURE COMMUNICATIONS (2020)

Article Biochemical Research Methods

Improvements of a high-throughput protein purification process using a calcium-dependent setup

Sara Kanje et al.

PROTEIN EXPRESSION AND PURIFICATION (2020)

Review Pharmacology & Pharmacy

The SARS coronavirus nucleocapsid protein - Forms and functions

Chung-ke Chang et al.

ANTIVIRAL RESEARCH (2014)

Article Biochemical Research Methods

High-throughput protein production - Lessons from scaling up from 10 to 288 recombinant proteins per week

Hanna Tegel et al.

Biotechnology Journal (2008)