Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 20, Issue 9, Pages 2957-2961Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2012.03.014
Keywords
Stopped-flow; Isoquinolinylboronic acid; Binding constant; Fluorescence
Funding
- National Institutes of Health [GM086925, GM084933]
- GSU University
- Harbin Medical University (PR China)
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The boronic acid group is widely used in chemosensor design due to its ability to reversibly bind diol-containing compounds. The thermodynamic properties of the boronic acid-diol binding process have been investigated extensively. However, there are few studies of the kinetic properties of such binding processes. In this report, stopped-flow method was used for the first time to study the kinetic properties of the binding between three model arylboronic acids, 4-, 5-, and 8-isoquinolinylboronic acids, and various sugars. With all the boronic acid-diol pairs examined, reactions were complete within seconds. The k(on) values with various sugars follow the order of D-fructose > D-tagatose > D-mannose > D-glucose. This trend tracks the thermodynamic binding affinities for these sugars and demonstrates that the 'on' rate is the key factor determining the binding constant. (C) 2012 Elsevier Ltd. All rights reserved.
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