Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 20, Issue 14, Pages 4443-4450Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2012.05.026
Keywords
Fluorescence; Ratiometric dye; Molecular rotor; Subcellular viscosity
Funding
- National Institutes of Health [1R21 RR025358, CA 133002]
- NSF [CHE-9709183, CHE-0741968]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0741968] Funding Source: National Science Foundation
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We describe the design, synthesis and fluorescence profiles of new self-calibrating viscosity dyes in which a coumarin (reference fluorophore) has been covalently linked with a molecular rotor (viscosity sensor). Characterization of their fluorescence properties was made with separate excitation of the units and through resonance energy transfer from the reference to the sensor dye. We have modified the linker and the substitution of the rotor in order to change the hydrophilicity of these probes thereby altering their subcellular localization. For instance, hydrophilic dye 12 shows a homogeneous distribution inside the cell and represents a suitable probe for viscosity measurements in the cytoplasm. (C) 2012 Elsevier Ltd. All rights reserved.
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