Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 20, Issue 2, Pages 601-606Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2011.06.041
Keywords
Activity-based protein profiling; Phosphonate probe; Paraoxon; Carboxylesterase; Nitrophenol; Arabidopsis thaliana
Funding
- Deutsche Forschungsgemeinschaft [KA 2894/1-1, HO 3983/3-2, HO3983/4-1]
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Activity-based protein profiling represents a powerful methodology to probe the activity state of enzymes under various physiological conditions. Here we present the development of a para-nitrophenol phosphonate activity-based probe with structural similarities to the potent agrochemical paraoxon. We demonstrate that this probes labels distinct serine hydrolases with the carboxylesterase CXE12 as the predominant target in Arabidopsis thaliana. The designed probe features a distinct labeling pattern and therefore represents a promising chemical tool to investigate physiological roles of selected serine hydrolases such as CXE12 in plant biology. (C) 2011 Elsevier Ltd. All rights reserved.
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