Journal
BRAZILIAN JOURNAL OF POULTRY SCIENCE
Volume 23, Issue 1, Pages -Publisher
FACTA-FUNDACIO ARNCO CIENCIA TECNOLOGIA AVICOLAS
DOI: 10.1590/1806-9061-2020-1378
Keywords
Chickens broilers; gut; Lactobacillus spp.; phenotypic identification; preservation
Categories
Funding
- Romanian Ministry of Education and Research [17 PFE/17.10.2018, PN 19.09.01.04]
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The study focused on isolating, identifying, and preserving Lactobacillus strains from the gut content of 45-day-old chickens broilers, as well as testing their viability at different temperatures. It was found that certain strains were suitable for further testing for probiotic traits.
The objective of this study was to isolate, identify, preserve and determine the quantitative level of the Lactobacillus strains from the gut content of 45-day-old chickens broilers; to test the viability of these strains preserved at 4 degrees C and room temperature (20 +/- 2 degrees C). Lactobacillus strains were isolated, phenotypically identified and preserved from the gut content of 17 chickens broilers. Identification was performed by morphological, cultural and biochemical characters examination, using apiwebTM and ABIS online software. The quantitative level of Lactobacillus strains in intestinal content (10(5) - 10(9) CFU/g) and the viability of strains preserved at 4 degrees C and at room temperature (from 8 days to 9 months) was also determined. Twenty-three strains of L. acidophilus, L. brevis, L. plantarum, L. fermentum and L. salivarius from the gut content of chickens broilers were isolated, phenotypically identified, and preserved. Of these, L. plantarum, L. fermentum and L. acidophilus biotype 1 strains were technologically and ecologically suitable to continue the testing of probiotic traits.
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