Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 19, Issue 5, Pages 1790-1801Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2011.01.012
Keywords
Proflavine; DNA-intercalation; Antitumor; Topoisomerase; Modeling
Funding
- Slovak Grant Agency VEGA [1/0053/08, 1/0476/08, 1/0097/10]
- State NMR Program [2003SP200280203]
- P.J. Safarik University in Kosice (VVGS) [7/09-10]
- NIH [P41 RR-01081]
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New acridine derivatives bearing two symmetrical imidazolidinone rings, 3,6-bis((1-alkyl-5-oxo-imidazolidin-2-yliden)imino)acridine hydrochlorides 6a-6e (alkyl = ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl), have been prepared and their interactions with calf thymus DNA and selected cell lines were studied. The DNA-binding of 6a-6e to ctDNA was examined by UV-vis, fluorescence, and CD spectroscopy. The binding constants determined by UV-vis spectroscopy were found in the range 1.9 x 10(5)-7.1 x 10(5) M-1. An electrophoretic separation proved that ligands 6a-6e inhibited topoisomerase I in 40 mu M concentration although only those with longer alkyl chains were able to penetrate the membranes and efficiently suppress the cell proliferation. The highest activity in cytotoxic tests was found for 3,6-bis((1-n-hexyl-5-oxo-imidazolidin-2-yliden) imino) acridine hydrochloride (6e) with IC50 = 2.12 mu M (HL 60) and 5.28 mu M (L1210) after 72 h incubation. Molecular dynamics simulations and calculations of solvent-accessible surface areas (SASAs) were used to explore the intercalation mechanism. MD simulations favor stacking between adjacent C: G base pairs from the minor groove side. MD and SASAs calculations indicate that the decrease of K with alkyl extension is due to negative entropic change upon binding. (C) 2011 Elsevier Ltd. All rights reserved.
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