Journal
NEW JOURNAL OF CHEMISTRY
Volume 45, Issue 10, Pages 4724-4728Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0nj05807f
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Funding
- Natural Science Foundation of China [21775096, 21878180]
- One hundred people plan of Shanxi Province
- Shanxi Province 1331 project key innovation team construction plan cultivation team [2018-CT-1]
- 2018 Xiangyuan County Solid Waste Comprehensive Utilization Science and Technology Project [2018XYSDJS-05]
- Shanxi Province Foundation for Returness [2017-026]
- Shanxi Collaborative Innovation Center of High Value-added Utilization of Coal-related Wastes [2015-10-B3]
- Shanxi Province 2019 annual science and technology activities for overseas students selected funding projects
- Innovative Talents of Higher Education Institutions of Shanxi
- Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi [2020L0541]
- Key R&D Program of Shanxi Province [201903D421069]
- Key R&D and transformation plan of Qinghai Province [2020-GX-101]
- Scientific Instrument Center of Shanxi University [201512]
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Hypochlorous acid, with low physiological concentration, is crucial in killing pathogens and has anti-inflammatory properties in the human immune system. Sensitively detecting tiny concentration changes of HOCl is necessary for understanding inflammation-associated diseases and cancer. The probe JBD effectively detects endogenous hypochlorous acid and provides insights into its role in oxidative stress and immune responses.
Hypochlorous acid (HOCl), with a low physiological concentration, plays a vital role in killing the pathogens and anti-inflammation in the human immune system. Deviation from the normal concentration is directly related to the inflammation-associated diseases and even cancer. Thus, it is necessary to sensitively detect the tiny concentration changes of HOCl. However, few of the reported probes could practically work well due to small Stokes shift, high detection of limits, and low SNR. In this study, HBT (2-(2 '-Hydroxyphenyl)benzothiazole) was used as the candidate for the ESIPT-based fluorophore and connected to pyridine via C = C bonds. The C = C bonds are the reaction site for HOCl and are activated by the strong electron-withdrawing CN group located on the double-bond carbon atom, resulting in a high sensitivity towards HOCl. Thereby, the probe's emission shifted effectively to the red channel avoiding the interference from biological autofluorescence. The excellent response of the probe JBD towards HOCl enables it to detect endogenous hypochlorous acid in the cells and provides an ideal molecular tool for exploring the mechanism of hypochlorous acid in oxidative stress and its immune role.
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