Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 16, Issue 20, Pages 9171-9175Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2008.09.024
Keywords
ORAC index; pyrogallol red; ascorbic acid; human blood plasma; human urine
Funding
- FONDECYT [11060323, 1070285]
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The oxygen radical absorbance capacity ( ORAC) methodology has been employed to estimate the antioxidant capacity of human blood plasma and human urine using pyrogallol red ( ORAC-PGR) as target molecule. Uric acid, reduced glutathione, human serum albumin, and ascorbic acid ( ASC) inhibited the consumption of pyrogallol red, but only ASC generated an induction time. Human blood plasma and human urine protected efficiently pyrogallol red. In these assays, both biological fluids generated neat induction times that were removed by ascorbate oxidase. From these results, ORAC-PGR method could be proposed as a simple alternative to evaluate an ORAC index and, simultaneously, to estimate the concentration of ascorbic acid in human blood plasma or human urine. (C) 2008 Elsevier Ltd. All rights reserved.
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