Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 116, Issue 10, Pages 2408-2418Publisher
WILEY
DOI: 10.1002/jcb.25235
Keywords
GROWTH FACTORS; TISSUE REGENERATION; ADIPOSE TISSUE; CYTOKINES
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Funding
- Associazione Italiana per la Ricerca sul Cancro (AIRC) [IG 12136]
- European Foundation for the Study of Diabetes (EFSD)
- Italian Minisrtry of Education (MIUR), MIUR-PRIN [2010MCLBCZ]
- Italian Minisrtry of Education (MIUR), MIUR-FIRB MERIT [RBNE08NKH7]
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Adipose tissue-derived mesenchymal stem cells (Ad-MSC) and platelet derivatives have been used alone or in combination to achieve regeneration of injured tissues. We have tested the effect of platelet-rich plasma (PRP) on Ad-MSC and adipocyte function. PRP increased Ad-MSC viability, proliferation rate and G1-S cell cycle progression, by at least 7-, 2-, and 2.2-fold, respectively, and reduced caspase 3 cleavage. Higher PRP concentrations or PRPs derived from individuals with higher platelet counts were more effective in increasing Ad-MSC growth. PRP also accelerated cell migration by at least 1.5-fold. However, PRP did not significantly affect mature adipocyte viability, differentiation and expression levels of PPAR- and AP-2 mRNAs, while it increased leptin production by 3.5-fold. Interestingly, PRP treatment of mature adipocytes also enhanced the release of Interleukin (IL)-6, IL-8, IL-10, Interferon-, and Vascular Endothelial Growth Factor. Thus, data are consistent with a stimulatory effect of platelet derivatives on Ad-MSC growth and motility. Moreover, PRP did not reduce mature adipocyte survival and increased the release of pro-angiogenic factors, which may facilitate tissue regeneration processes. (c) 2015 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals, Inc. J. Cell. Biochem. 116: 2408-2418, 2015. (c) 2015 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals, Inc.
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