Journal
BIOMOLECULES & THERAPEUTICS
Volume 16, Issue 4, Pages 394-402Publisher
KOREAN SOC APPLIED PHARMACOLOGY
DOI: 10.4062/biomolther.2008.16.4.394
Keywords
Prunus yedoensis; Atopic dermatitis (AD); MDC (CCL22); TARC (CCL17); JAK-STAT pathway; HaCaT keratinocytes
Funding
- Minister of Education & Human Resources Development, Republic of Korea
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Atopic dermatitis (AD) is an inflammatory skin disease commonly characterized by infiltration of inflammatory cells into skin lesions. Keratinocytes produce many chemokines that are involved in the pathogenesis of skin disorders. In particular, macrophage-derived chemokine (MDC/CCL22) and thymus and activation-regulated chemokine (TARC/CCL17) are Th2-type cytokines. Serum MDC and TARC levels are increased in AD patients. In this study, we investigated the anti-inflammatory effect and mechanism of action of the active fraction from Prunus yedoensis bark. We evaluated their inhibitory effects on the AD-like inflammatory markers (MDC: and TARC) and JAK-STAT pathway (STAT1) in HaCaT keratinocytes. The EtOAc fraction of the crude extract (80% EtOH) and the E5 sub-fraction potently inhibited the induction of MDC and TARC mRNA and protein at 50 mu g/mL in HaCaT cells. In addition, the E5 sub-fraction inhibited the phosphorylation of STAT1 protein associated with IFN-gamma signaling transduction in a dose-dependent manner. Thus, P. yedoensis may have anti-atopic activity by suppressing the inflammatory chemokines (MDC and TARC).
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