Journal
BIOMOLECULAR NMR ASSIGNMENTS
Volume 4, Issue 1, Pages 115-118Publisher
SPRINGER
DOI: 10.1007/s12104-010-9223-z
Keywords
RNA; NMR-assignments; Triple resonance experiments; Riboswitch; Aminoglycosides; Gene expression/regulation
Categories
Funding
- Center of Biomolecular Magnetic Resonance (BMRZ) of the Johann-Wolfgang-Goethe-University Frankfurt
- Deutsche Forschungsgemeinschaft (DFG) [WO 901/1-2, SU 402/4-1]
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The neomycin-sensing riboswitch is an engineered riboswitch developed to regulate gene expression in vivo in the lower eukaryote Saccharomyces cerevisiae upon binding to neomycin B. With a size of only 27nt it is the smallest functional riboswitch element identified so far. It binds not only neomycin B but also related aminoglycosides of the 2'-deoxystreptamine class with high affinity. The regulatory activity, however, strongly depends on the identity of the aminoglycoside. As a prerequisite for the structure determination of riboswitch-ligand complexes we report here the H-1, N-15, C-13 and partial P-31 chemical shift assignments for the minimal functional 27nt neomycin sensing riboswitch RNA in complex with the 4,5-linked neomycin analog ribostamycin and the 4,6-linked aminoglycoside tobramycin.
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