Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 838, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2022.156052
Keywords
Microfiltration; Ultrafiltration; Standard filtration mode; Antibiotic resistance genes; 16S rRNA gene; total cell counts
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Funding
- German Federal Ministry of Education and Research (BMBF) [02WRS137J]
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Low-pressure membrane filtration was studied for its removal of antimicrobial resistance genes in conventional secondary treated wastewater plant effluents. The pore size of the membrane was found to be the key factor influencing ARG removal, while the formation of fouling layer had significant effects on the removal of mobile ARGs. Different ARG abundances in feeds directly impacted the abundance of ARGs in the filtrate.
Low-pressure membrane filtration was investigated at pilot scale with regard to its removal of antimicrobial resistance genes (ARGs) in conventional secondary treated wastewater plant effluents. While operating microfiltration (MF) and ultrafiltration (UF) membranes, key operational parameters for antimicrobial resistance (AMR) studies and key factors influencing AMR removal efficiencies of low-pressure membrane filtration processes were examined. The main factor for AMR removal was the pore size of the membrane. The formation of the fouling layer on capillary membranes had only a small additive effect on intra-and extrachromosomal ARG removal and a significant additive effect on mobile ARG removal. Using feeds with different ARGs abundances revealed that higher ARG abundance in the feed resulted in higher ARG abundance in the filtrate. Live-Dead cell counting in UF filtrate showed intact bacteria breaking through the UF membrane. Strong correlations between 16S rRNA genes (as surrogate for bacteria quantification) and the sul1 gene in UF filtrate indicated ARBs likely breaking through UF membranes.
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