Journal
BIOMOLECULAR NMR ASSIGNMENTS
Volume 3, Issue 1, Pages 41-43Publisher
SPRINGER
DOI: 10.1007/s12104-008-9137-1
Keywords
Protein splicing; Intein; Protein trans-splicing
Categories
Funding
- Academy of Finland [118385]
- Sigrid Juselius Foundation
- Biocentrum Helsinki
- Academy of Finland (AKA) [118385, 118385] Funding Source: Academy of Finland (AKA)
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DnaE intein from Nostoc punctiforme (Npu) is one of naturally occurring split inteins, which has robust protein splicing activity. Highly efficient trans-splicing activity of NpuDnaE intein could widen various biotechnological applications. However, structural basis of the efficient protein splicing activity is poorly understood. As a first step toward better understanding of protein trans-splicing mechanism, we present the backbone and side-chain resonance assignments of a single chain variant NpuDnaE intein as determined by triple resonance experiments with [C-13,N-15]-labeled protein.
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