Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 117, Issue 3, Pages 780-792Publisher
WILEY
DOI: 10.1002/jcb.25368
Keywords
CHROMATIN REMODELING ENZYME; Chd5; RETROTRANSPOSON; STEM CELL BIOLOGY
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Funding
- Japan Society for the Promotion of Science (JSPS) [25116010, 26290064]
- Grants-in-Aid for Scientific Research [15K15365, 15K18457, 13J04024, 25116010, 25503003, 25840087, 24118002] Funding Source: KAKEN
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Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. (C) 2015 Wiley Periodicals, Inc.
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