Journal
BIOMICROFLUIDICS
Volume 3, Issue 4, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3267098
Keywords
biochemistry; bioelectric phenomena; biological effects of fields; cellular effects of radiation; electrokinetic effects; electrophoresis; microchannel flow
Categories
Funding
- NSF [CBET-0853873]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0853873] Funding Source: National Science Foundation
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Focusing cells into a single stream is usually a necessary step prior to counting and separating them in microfluidic devices such as flow cytometers and cell sorters. This work presents a sheathless electrokinetic focusing of yeast cells in a planar serpentine microchannel using dc-biased ac electric fields. The concurrent pumping and focusing of yeast cells arise from the dc electrokinetic transport and the turn-induced ac/dc dielectrophoretic motion, respectively. The effects of electric field (including ac to dc field ratio and ac field frequency) and concentration (including buffer concentration and cell concentration) on the cell focusing performance were studied experimentally and numerically. A continuous electrokinetic filtration of E. coli cells from yeast cells was also demonstrated via their differential electrokinetic focusing in a serpentine microchannel.
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