Gamma-Aminobutyric Acid A Receptor Subunit Expression and Cellular Localization in the Human Parkinsonian Globus Pallidus

BACKGROUND: The gamma-aminobutyric acid A (GABA(A)) receptor is an important mediator of cellular signaling in the globus pallidus and might be implicated in the pathophysiology of Parkinson disease (PD). The goal of the present study was to characterize GABA(A) receptor subunit expression in the normal and parkinsonian human globus pallidus. METHODS: Postmortem brain specimens were obtained from 8 patients with pathological evidence of PD at autopsy and from 4 control patients without such evidence. These tissues were exposed to primary antibodies directed against the alpha 1 and alpha 3 subunits of the GABA(A) receptor and were visualized and quantified using fluorescence microscopy. RESULTS: No differences were found in the pallidal neuronal density in the control versus PD tissues. Projection neurons strongly expressed the alpha 1, alpha 3, and beta 2 GABA(A) receptor subunits. After normalizing the immunofluorescence intensities in the globus pallidus to those in the adjacent structures, no significant differences were found in GABA(A) receptor subunit expression in the globus pallidus between the PD specimens and the control specimens. CONCLUSIONS: Compensatory changes in GABA(A) receptor alpha 1 and alpha 3 subunit expression in response to PD-related signaling abnormalities in the globus pallidus did not occur in our PD cohort.

Automated summary

The study aimed to characterize GABA(A) receptor subunit expression in the normal and parkinsonian human globus pallidus using fluorescence microscopy. The results showed no significant differences in GABA(A) receptor subunit expression between PD specimens and control specimens in the globus pallidus. Compensatory changes in GABA(A) receptor alpha 1 and alpha 3 subunit expression in response to PD-related signaling abnormalities in the globus pallidus were not observed in the PD cohort studied.