Spectrophotometric Determination Of Anti-Ulcer Drug (Cimetidine) By 2.4-Dinitrophenylhydrazine Reagent

An innovative, simple, and sensitive spectrophotometric method was described for the determination of cimetidine in both its pure form and in pharmaceutical formulations. By oxidizing 2.4-dinitrophenylhydrazine (2.4-DNPH) and mixing it with cimetidine in an alkaline solution, a brightly colored chromogen with a maximum absorption wavelength of 586 nm is created. After that, spectrophotometry was used to quantify the amount of cimetidine. Other analytical criteria, such as the ideal reaction circumstances, were evaluated. These criteria included variables like the volumes of 2.4-DNPH and potassium iodate, the amount of base, the coupling reaction time, temperature, and the order in which the constituents of the final product are added. Beer's law is applied to concentrations of (25-250) mu g/mL-1 with correlation coefficient (R2) = 0.9964, molar absorptivity 353.276 (L.mol-1.cm-1), and Sandell's law. The recommended method for locating cimetidine in pharmaceutical formulations worked well.

Automated summary

A new spectrophotometric method for determining cimetidine in pure form and pharmaceutical formulations, involving oxidation of 2.4-DNPH and coupling with cimetidine in alkaline solution to form a colored chromogen, was described and evaluated for various analytical criteria.