Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 20, Pages 6071-6079Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-8782-3
Keywords
High-internal-phase emulsion; Monolithic column; Solid-phase extraction; Cytokinins; Liquid chromatography-mass spectrometry
Funding
- National Natural Science Foundation of China [21265004, 21465008, 21065003]
- Natural Science Foundation from Guangxi Zhuang Autonomous Region [2014GXNSFAA118063, 2012GXNSFAA053030]
- project of high level innovation team and outstanding scholar in Guangxi colleges and universities
Ask authors/readers for more resources
High-internal-phase-emulsion polymers (polyHIPEs) show great promise as solid-phase-extraction (SPE) materials because of the tremendous porosity and highly interconnected framework afforded by the high-internal-phase-emulsion (HIPE) technique. In this work, polyHIPE monolithic columns as novel SPE materials were prepared and applied to trace enrichment of cytokinins (CKs) from complex plant samples. The polyHIPE monoliths were synthesized via the in-situ polymerization of the continuous phase of a HIPE containing styrene (STY) and divinylbenzene (DVB) in a stainless column, and revealed highly efficient and selective enrichment ability for aromatic compounds. Under the optimized experimental conditions, a method using a monolithic polyHIPE column combined with liquid chromatography-electrospray tandem mass spectrometry (LC-MS-MS) was developed for the simultaneous extraction and sensitive determination of trans-zeatin (tZ), meta-topolin (mT), kinetin (K), and kinetin riboside (KR). The proposed method had good linearity, with correlation coefficients (R (2)) from 0.9957 to 0.9984, and low detection limits (LODs, S/N = 3) in the range 2.4-47 pg mL(-1) for the four CKs. The method was successfully applied to the determination of CKs in real plant samples, and obtained good recoveries ranging from 68.8 % to 103.0 % and relative standard deviations (RSDs) lower than 16 %.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available