Preparation and characterization of an L-tyrosine-derivatized β-cyclodextrin-bonded silica stationary phase for liquid chromatography

An L-tyrosine-derivatized beta-cyclodextrin (CD)-bonded silica stationary phase (TCDS) for liquid chromatography (LC) was prepared by bonding L-tyrosine to beta-CD which had been grafted to the silica surface via a longer spacer. The new stationary phase was characterized using elemental analysis, and Fourier transform infrared (FT-IR) and solid state NMR spectroscopy. The separation of positional isomers, phenylpropionic acids and some dansyl amino acids was achieved on the new stationary phase. Their retention behavior was compared with that on the native beta-CD-bonded silica gel stationary phase. The results show that the introduction of L-tyrosine to beta-CD leads to enhancement of the retention of solutes such as p-nitrophenol and dansyl amino acids. (C) 2000 Elsevier Science B.V. All rights reserved.