Journal
VACCINE
Volume 18, Issue 11-12, Pages 1040-1050Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0264-410X(99)00347-3
Keywords
recombinant; vaccine; adenovirus
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A recombinant porcine adenovirus (rPAV) with the gp55 (E2) gene from the classical swine Fever virus (CSFV) 'Weybridge' strain inserted into the right hand end of the PAV serotype 3 (PAV3) genome was constructed. Expression of gp55 was directed by the major late promoter and tri-partite leader sequences located and cloned from PAV3. No compensatory deletions of PAV DNA sequences were made. Vaccination of outbred pigs with a single dose of the recombinant virus (rPAV-gp55) resulted in complete protection from lethal challenge with CSFV. No adverse clinical signs were observed in vaccinated animals following administration of rPAV-gp55 and following challenge, no clinical signs of CSF were observed prior to, or at, post mortem. The insert made into the rPAV increased the genome length to 106.8% of wild type and therefore exceeded the expected maximum insert size for a stable recombinant by almost 2%. Thus rPAV-gp55 contains the largest stable insertion made into a non-deleted Mastadeno virus recombinant so far reported. (C) 2000 Elsevier Science Ltd. All rights reserved.
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