Journal
TALANTA
Volume 50, Issue 6, Pages 1211-1222Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(99)00233-7
Keywords
iron speciation; milk; SEC-HPLC; ETAAS
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Speciation of iron in milk was carried out by high performance liquid chromatography (HPLC) and electrothermal atomic absorption spectrometry (ETAAS). Milk whey was obtained and low molecular weight protein separation was performed by size exclusion chromatography (SEC) with a TSK Gel SW glass guard (Waters) pre-column and a TSK-Gel G2000 glass (Toso Haas) column. After studying water as a possible mobile phase, this mobile phase was carefully selected in order to avoid alterations of the sample and to make subsequent iron determination in the protein fractions easier by ETAAS, The proposed method is sensitive (limit of detection [LOD] and LOQ 1.4 and 4.7 mu g 1(-1) respectively) and precise (relative standard deviation [RSD] < 10%). Iron is principally found in the proteins of 3 and 76 kDa in breast milk, and it is irregularly distributed in infant formulas. (C) 2000 Elsevier Science B.V. All rights reserved.
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