Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 233, Issue 1-2, Pages 13-20Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(99)00182-9
Keywords
purification; basophil; peripheral blood
Categories
Ask authors/readers for more resources
Studies of human basophils have been limited by the low number present in peripheral blood and the difficulties of purification to homogeneity with reasonable yield and functional status. Reproducible purification of human basophils to 96.5 +/- 0.5% with a yield of 40.8 +/- 5.3% was obtained by negative selection using immunomagnetic beads following initial separation by density gradient centrifugation. Isolated cells demonstrated complete viability by vital dye exclusion and spontaneous histamine release following incubation of <5%. Stimulation with anti-IgE or calcium ionophore A23187 caused histamine release and leukotriene C-4 production. Basophils demonstrated dose-dependent chemotaxis to monocyte chemotactic protein-3. This simplified methodology results in fully functional basophils in very high purity and good yield. (C) 2000 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available