Journal
JOURNAL OF IMMUNOLOGY
Volume 164, Issue 2, Pages 554-557Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.164.2.554
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Mycoplasmas and their membranes are potent activators of macrophages, the active principle being Lipoproteins and lipopeptides. Two stereoisomers of the mycoplasmal lipopeptide macrophage-activating lipopeptide-2 (MALP-2) differing in the configuration of the lipid moiety were synthesized and compared in their macrophage-activating potential, the R-MALP being >100 times more active than the S-MALP in stimulating the release of cytokines, chemokines, and NO, To assess the role of the Toll-like receptor (TLR) family in mycoplasmal lipopeptide signaling, the MALP-2-mediated responses were analyzed using macrophages from wild-type, TLR2-, TLR4-, and MyD88-deficient mice, TLR2- and MyD88-deficient cells showed severely impaired cytokine productions in response to R- and S-MALP, The MALP-induced activation of intracellular signaling molecules was fully dependent on both TLR2 and MyD88. There was a strong preference for the R-MALP in the recognition by its functional receptor, TLR2.
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