Journal
BRAIN RESEARCH
Volume 853, Issue 1, Pages 49-59Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0006-8993(99)02244-1
Keywords
microglia; P2 receptor; calcium signaling; receptor modulation
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Microglial cells are the immunocompetent cells of the CNS, which are known to exist in several activation states. Here we investigated the impact of microglial activation on the P2 receptor-mediated intracellular calcium ([Ca2+]ii) signaling by means of fluo-3 based Ca2+-imaging. Cultured mouse microglial cells were treated with either astrocyte-conditioned medium to induce a ramified morphology or LPS to shift the cells toward the fully activated stage. The extracellular application of ATP (100 mu M) induced a [Ca2+], elevation in 85% of both untreated and ramified microglial cells, whereas only 50% of the LPS-activated cells responded to the stimulus. To characterise the pharmacological profile of microglial P2 receptors we investigated the effects of various P2 agonists on [Ca2+](i) in cultured microglial cells. Untreated and ramified microglial cells demonstrated a very similar sensitivity to the different P2 agonists. In contrast, in LPS-activated microglia, a sharp decrease of responses to P2 agonist stimulation was seen. This indicates that microglial activation influences the capability of microglial cells to generate [Ca2+]i signals upon P2 receptor activation. (C) 2000 Elsevier Science B.V. All rights reserved.
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