Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 159, Issue 1-2, Pages 187-195Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/S0303-7207(99)00193-8
Keywords
thyroid hormone receptor; zebrafish; embryonic development; larvae
Categories
Ask authors/readers for more resources
The effects of thyroid hormones on metabolism and development are mediated by thyroid hormone receptors (TRs). We report the cloning and characterization of a TR beta 1 cDNA from zebrafish. Southern blot analysis revealed that there is a single genomic locus for the TR beta gene, while the TR alpha gene potentially has two loci. Multiple TR alpha and TR beta transcripts were detected in adult tissues. Using a semiquantitative RT-PCR assay, zygotic expression of TR alpha 1 and TR beta 1 were shown to occur before the midblastula transition stage. In transiently transfected HeLa cells, TR alpha 1 displayed constitutive transactivation in the absence of ligands, which was slightly enhanced by triiodothyronine (T3). The transactivating activity of TR beta 1 was strictly ligand-dependent and repressed in the absence of T3. Finally, the T3 induction of TR alpha 1 and TR beta 1 mRNAs was demonstrated in zebrafish embryos and larvae. The auto-induction of TR alpha 1 and TR beta 1 may serve a regulatory role during the embryonic and larval development of zebrafish. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available