Journal
BIOMETALS
Volume 25, Issue 4, Pages 749-759Publisher
SPRINGER
DOI: 10.1007/s10534-012-9543-9
Keywords
Inflammation; Hypoxia; Hepcidin; Adipocytes; Hepatocytes
Categories
Funding
- Diabetes and Endocrinology Society of Chile (SOCHED)
- FONDECYT [1085173, 1110080]
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Adipose tissue secretes numerous pro-inflammatory cytokines, such as interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha that can lead to insulin resistance (IR). In the liver, both IL-6 and TNF-alpha induce IR by inhibiting phosphorylation or ubiquitination of IRS1. In IR development, Fe is a risk factor in type-2 diabetes development. We studied the expression of genes related to inflammation, hypoxia, and mitochondrial function in hepatic (HepG2) and adipose (3T3-L1) cells. HepG2 and 3T3-L1 cells were incubated with 20 mu M Fe, 40 mu M Fe, or 40 mu M Fe/20 mM glucose for 7 days and then challenged with 20 ng/ml IL-6 and/or 100 mu M CoCl2 for 20 h. We measured intracellular Fe levels and the relative expression of hepcidin, NF-kappa B, IL-6, TNF-alpha, hypoxia inducible factor 1 alpha (HIF-1 alpha), and mitofusin 2 (Mfn-2) mRNA using qRT-PCR. The intracellular Fe concentration in HepG2 cells did not change with 20 or 40 mu M Fe. However, levels were decreased with Fe/glucose and IL-6 and/or CoCl2. 3T3-L1 cells showed an increase in intracellular Fe with high Fe plus either IL-6 or CoCl2. HepG2 cells incubated with 40 mu M Fe alone or Fe/glucose and challenged with IL-6 and/or CoCl2 showed increased IL-6, NF-kappa B, and TNF-alpha mRNA expression and decreased mRNA expression of Mfn-2 in all experimental conditions. 3T3-L1 cells incubated with 40 mu M Fe alone or Fe/glucose and challenged with IL-6 showed increased NF-kappa B mRNA expression and decreased Mfn-2 expression in all experimental conditions. Thus, high Fe, inflammation, and hypoxia trigger the expression of genes related to inflammation and Fe metabolism in HepG2 cells, in 3T3-L1 cells the same stimuli increased NF-kB and hepcidin expression.
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